R26-pVillin1-hCYP3A4-IRES-tdTomato(FVB)

Nomenclature
FVB-Gt(ROSA)26Sorem1(Gut-CYP3A4-IRES-tdTomato)/IL
Cat. NO.ILKI-18033
Strain StateEmbryo

Gene Summary:

SynonymsR26, ROSA26, AV258896, Gtrgeo26, Gtrosa26, Thumpd3as1
NCBI ID14910
MGI ID104735
Ensembl IDENSMUSG00000086429
Human OrthologsCYP3A4

Research Application:

Metabolism

Description:

Intestinal expression of the CYP3A enzyme in the human body can cause significant intestinal metabolism of the compound, resulting in impaired drug absorption. This knock-in model was generated by inserting the human CYP3A4 cDNA driven by the Villin1 promoter together with the IRES-tdTomato reporter gene into mosue Rosa26 site, which can be crossed with the Cyp3a13 gene knockout and other Cyp3a family genes knockout mice to obtain intestinal-expressed CYP3A4 humanized mouse model in order to determine the contribution of intestinal metabolism to the absorption and distribution of test article.