CRISPRi, gene regulation tool
R26-CAG-2xKRAB-dCas9-IRES-EGFP
R26-CAG-2xKRAB-dCas9-IRES-EGFP
Nomenclature
C57BL/6-Gt(ROSA)26Sorem1(CAG-Loxp-2xKRAB-dCas9-IRES-EGFP)/IL
Cat. NO.ILKI-18007
Strain StateLive
Gene Summary:
| Synonyms | R26, ROSA26, AV258896, Gtrgeo26, Gtrosa26, Thumpd3as1 |
| NCBI ID | 14910 |
| MGI ID | 104735 |
| Ensembl ID | ENSMUSG00000086429 |
| Human Orthologs |
Research Application:
Description:
These mice harbor a CAG-LSL-2xKRAB-dCas9-IRES-EGFP-WPRE-pA conditional expression cassette in mouse Rosa26 loci via homologous recombination. This strain can be used in CRISPR mediated target gene transcriptional inhibition (CRISPRi). The loxp-stop-loxp cassette was deleted.