Strategy Figures
Information of Knock-in Locus
Result of in vitro Transcription of Cas9 and gRNA
Figure 1: Agarose gel electrophoresis for in vitro transcribed Cas9 mRNA and guideRNA.
Genotyping Primer Sets for Identifying Homozygous, Heterozygous and Wild Type Mice
Figure 2. For genotyping, PCR followed by Sanger Sequencing was used to identify homozygous, heterozygous and wild type mice.
The Alignment of F1 Mice Sequencing Result
Genotyping Results
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