The endogenous mouse Il1b gene was replaced by human IL1B gene.
Validation Data
Figure 1. Detection of IL-1B expression in spleen and lung by RT-PCR. Wild type: only one band at 209 bp with primers F1/R1 (mIl-1b), Heterozygous: one band at 209 bp with primers F1/R1 (mIl-1b) and one band at 291 bp with primers F2/R2 (hIL-1B), Abbr.. M, DNA marker, HO, homozygous, HE, heterozygous, WT, wild type.
Figure 2. Detection of hIL1B expression in plasma by ELISA. Abbr. HO, homozygous, HE, heterozygous, WT, wild type. Note. hIL1B and Balb/c mice were i.p. injected with LPS(10mg/kg) for 6h.