The endogenous mouse Il9 gene was replaced by human IL9 gene.
Validation Data
Figure 1. Detection of IL-9 expression in thymus by RT-PCR.
Wild type: only one band at 159 bp with primers F1/R1 (mIl-9), Heterozygous: one band at 159 bp with primers F1/R1 (mIl-9) and one band at 162 bp with primers F2/R2 (hIL-9), Abbr.. M, DNA marker, HO, homozygous, HE, heterozygous, WT, wild type.
Figure 2. Expression characterization of hIL9 in hIL9 mice by ELISA.