The endogenous mice Gcgr gene was replaced by human GCGR gene.
Validation Data
Figure 1. Detection of GCGR expression in liver by RT-PCR.
Wild type: only one band at 261 bp with primers F1/R1 (mGcgr), Heterozygous: one band at 261 bp with primers F1/R1 (mGcgr) and one band at 223 bp with primers F2/R2 (hGCGR), Abbr.. M, DNA marker, HO, homozygous, HE, heterozygous, WT, wild type.
Figure 2. Detection of human GCGR expression in liver in hGCGR knockin mice by IHC.
Liver were collected from wild-type mice (+/+) and homozygous hGCGR mice (HO/HO), and analyzed by IHC with anti-human GCGR antibody.