The endogenous mice Gdf15 gene was replaced by human GDF15 gene.
Validation Data
Figure 1. Detection of GDF15 expression in intestine and colon and liver by RT-PCR.
Wild type: only one band at 314 bp with primers F1/R1 (mGdf15), Homozygous: only one band at 160 bp with primers F2/R2 (hGDF15), Abbr.. M, DNA marker, HO, homozygous, HE, heterozygous, WT, wild type.
Figure 2. Detection of hGDF15 expression in plasma by ELISA.
Abbr. HO, homozygous, HE, heterozygous, WT, wild type.
Figure 3. Detection of mGDF15 expression in plasma by ELISA.
Abbr. HO, homozygous, HE, heterozygous, WT, wild type.