Immunotherapy, cancer research, drug screening
IL2 & IL2RA
IL2 & IL2RA
Nomenclature
C57BL/6-Il2tm3(hIL2)Il2ratm1(hIL2RA)/IL
Cat. NO.ILHU-200275
Strain StateEmbryo
Gene Summary:
| Synonyms | Il-2, CD25, Il2r, IL-2R alpha chain, Ly-43 |
| NCBI ID | 16183, 16184 |
| MGI ID | 96548, 96549 |
| Ensembl ID | ENSMUSG00000027720, ENSMUSG00000026770 |
| Human Orthologs | IL2, IL2RA |
Research Application:
Description:
IL2-HU (ILHU-190048) was crossed with IL2RA-HU (ILHU-190064) to generate humanized IL2 &, IL2R mice.
Validation Data
Figure 1. Detection of hIL-2 expression by ELISA in the hIL-2 knock-in mice (Wild type and heterozygous hIL-2 KI mice were administrated with concanavalin).
Figure 2. Intracellular staining of IL-2 by FACS post stimulation with anti-mCD3/anti-mCD28 (Data in partnership with collaborators).
Figure 3. IL-2RA humanized mouse expresses hlL-2RA (hCD25) on naïve Treg in blood (Data in partnership with collaborators).
Figure 4. hlL-2RA is highly expressed on activated Tregs in spleen in the IL-2RA humanized mouse (Data in partnership with collaborators).
Figure 5. hlL-2RA is highly expressed on activated NK cells in spleen in the IL-2RA humanized mouse (Data in partnership with collaborators).