The endogenous mouse Il21 gene was replaced by human IL21 gene.
Validation Data
Figure 1. Detection of IL21 expression in splenocyte by RT-PCR.
Wild type: only one band at 272 bp with primers F1/R1(mIl21), Homozygous: only one band at 304 bp with primers F2/R2(hIL21), Abbr. M, DNA marker, HO, homozygous, HE, heterozygous, WT, wild type.
Figure 2. Detection of hIL21 expression in splenocyte culture supernatants by ELISA (n=3).
Abbr. HO, homozygous, HE, heterozygous, WT, wild type. Note. hIL21 and C57BL/6 mice were stimulated with ConA in vitro.