Cytokine humanization model, drug screening
IL33
IL33
Nomenclature
C57BL/6-Il33tm2(hIL33)/IL
Cat. NO.ILHU-2000069
Strain StateEmbryo
Gene Summary:
| Synonyms | Il-33, Il1f11, NF-HEV, 9230117N10Rik |
| NCBI ID | 77125 |
| MGI ID | 1924375 |
| Ensembl ID | ENSMUSG00000024810 |
| Human Orthologs | IL33 |
Research Application:
Description:
The endogenous mouse Il33 gene was replaced by human IL33 gene.
Validation Data
Figure1. Detection of IL33 expression in lung by RT-PCR. Wild type: only one band at 287 bp with primers F1/R1(mIl33),Homozygous: only one band at 311 bp with primers F2/R2(hIL33), Abbr.. M, DNA marker, HO, homozygous, HE, heterozygous, WT, wild type.
Figure 2. Detection of hIL33 expression in lung homogenate by ELISA. Abbr. HO, homozygous, HE, heterozygous, WT, wild type. Note. hIL33 and C57BL/6 mice were i.p. injected with LPS (10mg/kg) for 4h.
Figure 3. Detection of mIL33 expression in lung homogenate by ELISA. Abbr. HO, homozygous, HE, heterozygous, WT, wild type. Note. hIL33 and C57BL/6 mice were i.p. injected with LPS (10mg/kg) for 4h.