Cytokine humanization model, drug screening
IL12B
IL12B
Nomenclature
C57BL/6-Il12betm3(hIL12B)/IL
Cat. NO.ILHU-2000057
Strain StateEmbryo
Gene Summary:
| Synonyms | p40, Il-12b, Il12p40, Il-12p40 |
| NCBI ID | 16160 |
| MGI ID | 96540 |
| Ensembl ID | ENSMUSG00000004296 |
| Human Orthologs | IL12B |
Research Application:
Description:
The endogenous mouse Il12b gene was replaced by human IL12B gene.
Validation Data
Figure 1. Detection of IL12B expression in thymus by RT-PCR. Wild type: only one band at 260 bp with primers F1/R1 (mIl12b), Heterozygous: one band at 260 bp with primers F1/R1 (mIl12b) and one band at 260 bp with primers F2/R2 (hIL12B), Abbr.. M, DNA marker, HO, homozygous, HE, heterozygous, WT, wild type.
Figure 2. Detection of hIL12B expression in plasma by ELISA. Abbr. HO, homozygous, HE, heterozygous, WT, wild type. Note. hIL12B and C57BL/6 mice were i.p. injected with LPS (10mg/kg) for 2h.
Figure 3. Detection of mIL12B expression in plasma by ELISA. Abbr. HO, homozygous, HE, heterozygous, WT, wild type. Note. hIL12B and C57BL/6 mice were i.p. injected with LPS (10mg/kg) for 2h.