Immunotherapy, cancer research, drug screening
IL2RA
IL2RA
Nomenclature
C57BL/6-Il2raem1(hIL2RA)/IL
Cat. NO.ILHU-190064
Strain StateLive
Gene Summary:
| Synonyms | CD25, Il2r, IL-2R alpha chain, Ly-43 |
| NCBI ID | 16184 |
| MGI ID | 96549 |
| Ensembl ID | ENSMUSG00000026770 |
| Human Orthologs | IL2RA |
Research Application:
Description:
The endogenous mouse Il2ra gene was replaced by human IL2RA gene.
Validation Data
Figure 1. Detection of IL2RA expression by RT-PCR.
Wild type: only one band at 203 bp with primers F2/R2 (mIl2ra), Homozygous: only one band at 251 bp with primers F1/R1 (hIL2RA), Abbr.. M, DNA marker, HO, homozygous, HE, heterozygous, WT, wild type.
Figure 2. Development and validation of hIL2RA knockin mice. (Data in partnership with collaborators)
(A) Targeting strategy for hIL2RA knockin mice development, (B) hIL2A expression on naive Tregcells in blood in homozygous hlL2RA knockin mice, (C) hlL2A expression on naive Treg cells in spleen in homozygous hlL2RA knockin mice.
Figure 3. Characterization of hIL2RA knockin mice. (Data in partnership with collaborators)
(A) hlL-2RA is highly expressed on activated Tregsin spleen in IL-2RA humanized mouse, (B) hlL-2RA is highly expressed on activated NK cells in spleen in IL-2RA humanized mouse.
Figure 4. In vivo efficacy study in hIL-2RA knockin mice engrafted with MC38 syngeneic tumor model. (Data in partnership with collaborators)
(A) Tumor growth curves upon treatment, (B) Body weight change upon treatment.