PD-L1 & CTLA4

Nomenclature
C57BL/6-Ctla4em1(hCTLA4)Cd274em1(hPD - L1)/IL
Cat. NO.ILHU-00102
Strain StateEmbryo

Gene Summary:

SynonymsB7h1, Pdl1, Pdcd1l1, Pdcd1lg1, A530045L16Rik, Cd152, Ly-56, Ctla-4
NCBI ID12477, 60533
MGI ID88556, 1926446
Ensembl IDENSMUSG00000026011, ENSMUSG00000016496
Human OrthologsCTLA4, CD274

Research Application:

Immunotherapy, cancer research, drug screening

Description:

Generation Strategy

Strategy for Generating Humanized PD-L1 (ASHU-00062) Mouse Model

landingpage-ashu-00102-PD-L1 &amp, CTLA4-hu-icpmouse-1.1

Strategy for Generating Humanized CTLA4 (ASHU-00014) Mouse Model

landingpage-ashu-00102-PD-L1 &amp, CTLA4-hu-icpmouse-2

Validation Data

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Figure 1. Splenocytes from homozygous hPD-L1/hCTLA4 mice were analyzed by flow cytometry after activation. The murine Pd-l1 (mPD-L1) and Ctla4 (mCTLA4) positive cells were detectable in wild type (WT) C57BL/6 mice, while the human (hPD-L1) and human (hCTLA4) positive cells were detectable in the homozygous hCTLA4/hPD-L1 mice.

landingpage-ashu-00102-PD-L1 &amp, CTLA4-hu-icpmouse-4
landingpage-ashu-00102- PD-L1 &amp, CTLA4-hu-icpmouse-4

Figure 2. hCTLA-4/PD-L1 mice+ MC38 cells efficacy studies. We have developed a CTLA-4/PD-L1 dKI humanized model and tested the combined treatment of Yervoy and Tecentriq, and then observed improved efficacy in the combination treatment.