The Cyp2e1 is a member of the CYP2 subfamily of cytochrome P450 enzymes which is responsible for many reactions involved in drug metabolism, synthesis of cholesterol, steroids and other lipids in humans. The Cyp2e1 is mainly expressed in hepatocytes and plays an important role in alcohol and toxin metabolism.
The Cyp2e1-CreERT2 mouse model was generated using CRISPR/Cas9 and homology directed repair (HDR) where the CreERT2 recombinase sequence was inserted downstream of the Cyp2e1 5’ UTR in the mouse endogenous gene locus and its expression is controlled by the Cyp2e1 promoter. This mouse model enables the generation of liver-specific tamoxifen-inducible conditional knockout or expression mouse model.
Validation of Humanized Mouse Model
Knock-in Strategy
Figure 1. Gene knock-in strategy to engineer Cyp2e1-CreERT2 mouse line. Briefly